LOW DOSE STREPTOZOTOCIN-INDUCED DIABETES  IN WISTAR RATS IS ASSOCIATED WITH REDUCED  INSULIN   IMMUNOREACTIVE PANCREATIC    ISLETS

Ouali, K.; Bairi, A.; Trea, F.; Frih, H.; Siaud, P.; Guellati, Ma.

Synthèse
Volume 11, Numéro 1, Pages 1-8
2005-06-30

Low Dose Streptozotocin-induced Diabetes In Wistar Rats Is Associated With Reduced Insulin Immunoreactive Pancreatic Islets

Authors : Ouali K. . Bairi A. . Trea F. . Frih H. . Siaud P. . Guellati Ma. .

Abstract

Streptozotocin, which induces diabetes mellitus in laboratory animals, has been reported to be interred into by B- cells by means of the glucose transporter 2 (GLUT2) and then reduced the cellular level of NAD+ leading to necrosis of the ~-cells. In this experiment we investigated the effect of low dose streptozotocin (50 mg/kg) on insulin immunoreactivity in pancreatic islets. Two groups of rats were studied , the first group received streptozotocin (STZ) and the second was used as a control. Rats treated with STZ became gradually hyperglycemic on day 21 STZ: 318.8 ± 24.2 mg/dl vs CTRL: 65.1 ± 8.2 mg/dl; P< 0.001 with sinificant reduction of plasma insulin 6.3 ± 1.36 f.!UIIml vs CTRL 22.4 ± 2.8 ul.ll/rnl; P< 0.01). The immunohistochemical findings of insulin IR in the pancreatic tissue were in accordance with the results obtained for glucose and plasma insulin. In conclusion, we can say. That a single low dose of STZ provoke destruction of pancratic ~-cells and the diabetogenic action of this drug is probably mediated by GLUT2