Applied Biology in Saharan Areas
Volume 3, Numéro 4, Pages 10-26
2021-12-31
Makh.ah75@gmail.com
Authors : Makhloufi Ahmed . Mezouari Amina . Manuel González Díaz Jesús . Benlarbi Larbi .
Abstract
Antimicrobial activity of medicinal plants have been recognized for a long time. This study has been concerned with determining the chemical composition and antimicrobial activity of Tar extracted from Accacia radiana subsp.radiana wood growing wild in Bechar region, (South West of Algeria). This tar was extracted by traditional method and then was analyzed by gas chromatography coupled with mass spectroscopy technique (GC-MS technique) and calculation of Kovats Retention Indices values. Exactly 69 components were recognized that components having the highest percent are as follow: Syringol (6.86%),2,6-Dihydroxy-4-methoxyacetophenone(5.94%) ,2,3-Dimethoxybenzyl alcohol (5.06%), p-Ethylguaiacol (3.68%). Antimicrobial effects of this tar were tested against six strains of bacteria and five strains of fungi. All strains were inhibited (Minimal Inhibition Concentration)at the concentration of 0.1mg/ml, Escherichia coli ATCC 25922 and Enterococcus feacalis ATCC 29212 were the most sensitive with CMI of 0.008 mg/ml. for Fungi, the Tar shows that the best CMI(0.006 mg/ml) was obtained against Alternaria alternata and Fusarium oxysporum f. sp. albedinis. Keywords: Accacia tortilis subsp.radiana, Tar, Chemical Composition, Antimicrobial activity, Bechar.
Keywords
Accacia tortilis subsp.radiana ; Tar ; Chemical Composition ; Antimicrobial activity ; Bechar.
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