Journal of Advanced Research in Science and Technology
Volume 5, Numéro 1, Pages 640-647
2018-06-01

Influence Of Temperature On Antibiotic Susceptibility Testing Of Escherichia Coli Ho25 By The Agar Diffusion Method



Authors : Saïd Abdelkrim .

Abstract

In this study, we evaluated the influence of temperature on the antibacterial activity against Escherichia coli HO25 by measuring the inhibition zone diameters on a agar diffusion method. The antibiotic sensitivity profile was determined by testing four values of temperatures: 25, 30, 37 and 44 °C. The results obtained, indicated that there is no significant difference between 30 and 37 °C (Tukey test). Consequently, the temperature 37 °C could be replaced by 30 °C in medical and research laboratories for agar diffusion method. Furthermore, we observed that except gentamicin, all antibiotics used showed no change in resistance profile by using different temperatures.

Keywords

temperature ; antibiotic ; Escherichia coli HO25 ; antibiogram, ; agar diffusion method

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