AGROBIOLOGIA
Volume 12, Numéro 2, Pages 3267-3275
2022-12-31

On Mosquito (diptera: Culicidae) Agent Of Pathogenic Diseases.



Authors : Kirouani Mouna . Chebouti-meziou Nadjiba Nadjiba . Chebouti Yahia .

Abstract

Description of the Subject: As part of the search for a biological method against Culicidae vectors of pathogenic diseases, sensitivity tests are carried out on mosquitoes by the use of polyphenolic extract of pistachio: Pistacia terebinthus, Pistacia lentiscus and Pistacia atlantica. Objective: This study contributes to the enhancement of plant extracts of the Pistacia genera. To this end,a reduction in the use of chemicals, as well as their imports; and finally to use an effective, natural and economical means of control. Methods: In this work, the insecticidal activity of these extracts was studied on stage 1 and 4 larvae of Culex pipiens (Linnaeus). Toxicity tests revealed after 24/48 and 72 hours and after one week to determine LC50 and TL50, the results are confirmed by the ANOVA and Tukey test. Result: In general, the polyphenolic extract has a strong larvicidal effect, in particular the polyphenolic extract of Pistacia lentiscus and Pistacia atlantica. Our results showed a very interesting larvicidal activity, with LD50 values ​​of Pistacia lentiscus of 30.44 mg / ml and 92.30 mg / ml of L1 and L4 larval stages after 24 hours. On the other hand, the toxicity is well marked when the duration of exposure of the larvae is longer with a mortality rate reaching 100% for L1 and L3 after 72 hours for the polyphenolic extract of Pistacia lentiscus and Pistacia atlantica. The results show that the polyphenolic extract of the leaves of Pistacia lentiscus and Pistacia atlantica has a very significant toxic effect, is p = 0.000 for the different larval stages, Pistacia lentiscus and Pistacia atlantica have a remarkable larvicidal activity. Conclusion: As part of the fight against mosquitoes, the polyphenolic extract of the leaves of three plants of the genus Pistacia is used as a natural biocide.

Keywords

larvicidal activity; ; polyphenolic extract; ; Pistacia lentiscus; ; Pistacia atlantica; ; Pistacia vera; ; Culex Pipiens.

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