Revue des Sciences Fondamentales Appliquées
Volume 9, Numéro 1, Pages 392-410
2017-01-01
Authors : Ghouila Z. . Laurent S. . Boutry S. . Vander Elst L. . Nateche F. . Muller R. N. . Baaliouamer A. .
In this work and for the first time, significant concentrations of total polyphenols and flavonoids from Vitis vinifera L. grape seed extracts were obtained (256.15 ± 17.40 mg GAE/gdm and 14.08 ± 0.64 mg CE/gdm, respectively).The LC/MS analysis revealed richness in procyanidins. For antioxidant, antimicrobial and antifungal effects, the grape seed extract (GSE) responded positively. At 100 μg/mL, GSE induced a moderate toxicity of the order of 3.88% on 3T6 cells at the first 24 hours of treatment, whereas, its prolonged effect to 48 hours reduced this toxicity to less than 0.5%. As for the anti-proliferative effect on tumoral cell lines, a cell death of 18.39% to 23.79% and 10.30% to 20.37% was registered respectively for HeLa and BCPAP cells during 24 and 48 hours of treatment. Consequently, it is possible to consider using GSE at lower concentrations as an anti-proliferative agent without losing sight of its benefic effect on healthy cells.
grape seed extract, 3T6 cell, antioxidant, antibacterial, anti-proliferation.
Ghouila Z.
.
Laurent S.
.
Henoumont C.
.
Vander Elst L.
.
Muller R. N.
.
Baaliouamer A.
.
pages 692-711.
Ait Ouahioune Lidia
.
Bara Fatma
.
Bariz Karim
.
Houali Karim
.
Djenane Djamel
.
pages 298-308.
Chouikh Atef
.
Rebiai Abdelkrim
.
Aref Mahdia
.
Heded Mounira
.
Adjal El Hadda
.
Alia Fatma
.
pages 14-17.
Belmimoun A.
.
Meddah B.
.
Meddah A.t.
.
Sonnet P.
.
pages 510-524.
Benmeziane Farida
.
Cadot Yves
.
pages 201-205.