Synthèse
Volume 26, Numéro 2, Pages 25-32
2020-10-20
Influence Of Rhus Tripartita (ucria) Grande Leaves On Phyto-beneficial Bacteria Associated With Its Rhizosphere
Authors : أسماء عيودة . Benaissa Asmaa . Djebbar Réda . Abderrahmani Ahmed .
Abstract
Leaves extracts were performed using distilled water, alcohol, methanol, hexane and chloroform as solvent and diluted in concentrations of 0.001, 0.01 and 0.1 mg/mL. Meanwhile, the extraction of total flavonoids was carried out according to the standard procedure. The antimicrobial effect of the extracts was evaluated using the agar diffusion method and the determination of the minimum inhibitory concentration was carried out on a liquid medium. Alcohol, chloroform and methanol extracts were found to be the most effective on tested strains. The maximum zone inhibition was 18 mm, and the minimum zone inhibition was 7 mm. Bacillus licheniformis (RT 1) appears to be the most sensitive to all extracts. In contrast, Bacillus megaterium (RT 7) seems to be the less sensitive strain. On the other hand, total flavonoids had a significant effect on 25 % of the strains tested, espicialy Bacillus genus. With a broad antimicrobial spectrum, the Rhus tripartita leaves can be considered as a control agent for the distribution of the bacterial community in the rhizosphere. Therefore, this study showed that the plant could influence the bacterial diversity of its rhizosphere through its leaves.
Keywords
Rhus tripartita, flavonoids, leaves, PGPR antagonist, rhizosphere.
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